From Biology to Nanotechnology - a Biomimetic Approach
Mar/06/2009 11:16 Filed in: Seminars
Electro-Optics/Physics Seminar
From Biology to Nanotechnology - a Biomimetic Approach
Dr. Jöerg Opitz
Head, Optical Diagnosis Department
Fraunhofer Institute for Non-Destructive Testing Dresden
Friday, March 6, 2009
3:00 pm - KL 405
All are invited
From Biology to Nanotechnology - a Biomimetic Approach
Dr. Jöerg Opitz
Head, Optical Diagnosis Department
Fraunhofer Institute for Non-Destructive Testing Dresden
Friday, March 6, 2009
3:00 pm - KL 405
All are invited
Head, Optical Diagnosis Department
Fraunhofer Institute for Non-Destructive Testing Dresden
The aim of this talk is to show activities in the field of BioNanotechnology in the region of Dresden, Germany. For this two specific topics are selected.
Recent investigations in the development of novel strategies for a controlled bottom-up fabrication of artificial nanostructures with tailored quantum properties involve synthesis routes that make use of self-assembly and molecular recognition capabilities of biomolecules. In particular, DNA can be considered as basic building block for nanostructure fabrication because the specific Watson-Crick base pairing allows to program their intra- and intermolecular associations and thus to build-up artificially designed supramolecular structures. Moreover, DNA constitutes an ideal template for the organization of metals into wire-like assemblies for nanoelectronic applications. This talk addresses the issue of controlled integration of single DNA molecules into microelectronic contact arrays, which involves site-specific binding of single DNA molecules and their manipulation in hydrodynamic flow and with motor proteins.
For the investigation of biomolecular processes in- and outside of cells there is a steadily increasing importance of fluorescent biomarkers. Many different fluorescent biomarkers are available, but most of them do not fulfil all of the required physical and optical properties. Recent studies show nanodiamonds as promising red fluorescent biomarkers. Nanodiamonds seem to surpass organic dyes and fluorescent proteins in terms of photo bleaching properties and quantum dots in terms of cell cytotoxicity. In this study optical properties of green fluorescent nanodiamonds of a diameter of about 25 nm will be presented. As a proof of concept we modified the surface of the nanodiamonds with antibodies to biofunctionalise them for specific targeting to biomolecules. We show the successful transfection of nanodiamond immunoconjugates in JIMT-1and HeLa cells.
Fraunhofer Institute for Non-Destructive Testing Dresden
The aim of this talk is to show activities in the field of BioNanotechnology in the region of Dresden, Germany. For this two specific topics are selected.
Recent investigations in the development of novel strategies for a controlled bottom-up fabrication of artificial nanostructures with tailored quantum properties involve synthesis routes that make use of self-assembly and molecular recognition capabilities of biomolecules. In particular, DNA can be considered as basic building block for nanostructure fabrication because the specific Watson-Crick base pairing allows to program their intra- and intermolecular associations and thus to build-up artificially designed supramolecular structures. Moreover, DNA constitutes an ideal template for the organization of metals into wire-like assemblies for nanoelectronic applications. This talk addresses the issue of controlled integration of single DNA molecules into microelectronic contact arrays, which involves site-specific binding of single DNA molecules and their manipulation in hydrodynamic flow and with motor proteins.
For the investigation of biomolecular processes in- and outside of cells there is a steadily increasing importance of fluorescent biomarkers. Many different fluorescent biomarkers are available, but most of them do not fulfil all of the required physical and optical properties. Recent studies show nanodiamonds as promising red fluorescent biomarkers. Nanodiamonds seem to surpass organic dyes and fluorescent proteins in terms of photo bleaching properties and quantum dots in terms of cell cytotoxicity. In this study optical properties of green fluorescent nanodiamonds of a diameter of about 25 nm will be presented. As a proof of concept we modified the surface of the nanodiamonds with antibodies to biofunctionalise them for specific targeting to biomolecules. We show the successful transfection of nanodiamond immunoconjugates in JIMT-1and HeLa cells.
